Application of simultaneous Equation Method for the Determination of Azithromycin and Cefixime Trihydrate in Tablet Formulation

A simple, accurate, and precise uv-spectrophotometric method has been developed for the simultaneous estimation of azithromycin (AZI) and cefixime trihydrate (CEFI) in tablet formulation. The method was based on employing simultaneous equation method for the analysis of both drugs. AZI and CEFI have shown absorbance maxima at 222 and 289 nm in methanol, respectively. The linearity was obeyed in the concentration range of 10-50µg/ml for both drugs, with a significantly high correlation coefficient (r2 = 0.999). The limits of detection for AZI and CEFI were 0.81 and 1.52 µg/ml, respectively, and the limits of quantitation for AZI and CEFI were 2.40 and 4.60 µg/ml, respectively. The suitability of the developed method for quantitative determination of drugs was proved by validation. The method was successfully used to analyze a tablet formulation.