The usability of quantification of myocardial blood flow by cardiac magnetic resonance for evaluation of cell therapy for ischemic cardiomyopathy using a retrograde injection method

Methods The candidate of this study was 5 cases of ICM (All Male, 64.3 + 7.7 y/o). Bone marrow Mononuclear cells (BMMNC) were quickly injected retrogradely via the coronary vein into the myocardium of LAD area through the infusion catheter. The number of injected BMMNC is 1.47 + 0.35 × 108. P-MRI and SPECT were performed before and after procedure. P-MRI was obtained with a saturation recovery balanced TFE sequence, by injecting 0.05 mmol/ kg of Gd-DTPA during vasodilator stress and at rest. Dual bolus method was used to correct for blood saturation. Absolute MBF was quantified by using a model based Patlak analysis, with correction for flow-dependent alteration in extraction fraction of Gd-DTPA. Therapy evaluation were made, which were 1) Symptom of Angina pectoris with CCS scoring. 2) SPECT score: Summed rest Score (SRS), Summed stress score (SSS), Summed Difference Score (SDS). 3) BMF and Coronary Flow Reserve (CFR) from P-MRI.