PECULIARITIES OF UNITIOL INFLUENCE ON THE KIDNEY FUNCTIONING IN RATS OF THE JUVENILE AGE

This investigation shows an influence of different dosages of unitiol (sodium2,3-dimercaptopropanesulfonate) on the kidney functioning in juvenile rats.Purpose of the work is to study unitiol influence upon the kidney excretion function insexually mature rats at single and repeated injections.Material and methods. This investigation was performed on the juvenile male rats withthe body weight 95.0±8.5 g. The kidney functioning was determined for the single andrepeated (10, 20 and 30 days) injections of 5 % unitiol solution (50 mg/kg, subcutaneously)against a background of the additional water load realized through introduction of warmwater into the stomach in the volume of 5 % of the animal’s body weight. The concentrationOriginal research53Клінічна та експериментальна патологія. 2020. Т.19, №1(71) ISSN 1727-4338 https://www.bsmu.edu.uaof sodium, potassium ions, creatinine, proteins, ammonium, titratable acids and pH weredetermined in the rat’s urine and then compared with the corresponding values obtainedfrom the control and previous groups under study.Results. No significant changes were determined in the excretion and acid-regulationfunctions of the rat’s kidney in 2.5 hours after unitiol single injection against a backgroundof the water load. However, the concentration of sodium increased 1.7 times as large andthe concentration of potassium decreased correspondingly. No changes were registeredin diuresis while excretion of sodium increased 1.9 times as large, kaliuresis decreased1.6 times, sodium/potassium urine coefficient increased 2.6 times.After daily untiol injection during 10 days, concentration of sodium in the urine became3.2 times less in comparison with animal’s reaction to the single injection of the drugand 1.9 times less than that in the control group. Against a background of the 20 and30 days of the drug injection, the concentration of sodium has decreased almost thricecomparing to the control group value. Natriuresis decreased by 4 times comparing tothe value achieved after a single drug injection, decreased twice in comparison withthe control group and then it remained unchanged throughout the following 20- or 30-days long injections. No significant deviations from the control value were found in thepotassium concentration after the repeated injections of the drug. Kaliuresis decreasedafter the single injection but then it increased to its control value following the injection ofthe drug during 20 days. The urine sodium/potassium coefficient decreased 1.7-2.5 timescomparing to the control value after the repeated injections. No significant changes wereregistered throughout the entire experiment in the endogenous creatinine excretion andconcentration. The protein concentration decreased by 2.2 times comparing to its controllevel after unitiol application during 10 days and then it remained unchanged after 20-or 30-days long courses of injections. The indices of titratable acids and ammoniumexcretion became in 1.8 times less than those in the control group, then they increasedafter the 20th day and on the 30th day of injection they didn’t significantly differ fromthe control indices. The hydrogen excretion increased 2.4 and 2.8 times as large after20 and 30 days of the drug injection respectively at urine pH decrease. Unlike the singleinjection, the repeated unitiol injections significantly decrease natriuresis and the proteinexcretion with urine. Some age-related features of unitiol influence on the kidneys can beaccounted at different types of its administration as a chemical antidote.Conclusions. 1. Unitiol influence on the kidney functioning combined with the water loadin juvenile rats depends on the duration of application.2. The natriuretic effect increases and the kaliuretic effect decreases after a singleinjection of the drug when there is no significant influence on diuresis, excretion andacid-regulating parameters of the kidney. 3. A prolonged (10, 20, 30 days) application ofunitiol causes some intensification of sodium-retaining and hypoproteinuretic activitiescombined with some increase in the urine pH.