Preparation method of redox probe for marking h-IGg (Human-Immunoglobulin G) impedance immunosensor

The present invention discloses a method for preparing a non-reducing probes labeled h-IGg impedance immunosensor for oxidation. Have absolute ethanol to 3,5-dibromo-salicylaldehyde dissolved in absolute ethanol was added dropwise a solution containing β- mercaptoethylamine, water bath, recrystallized, and dried in vacuo to yield β- mercaptoethylamine condensing 3 3,5-dibromo-salicylaldehyde Schiff base; anhydrous ethanol and N, N'- dimethylformamide volume ratio of 2: 1 mixed solvent made, to a solution of 3,5-condensing β- mercaptoethylamine a mixed solvent dibromo salicylaldehyde Schiff base containing nickel acetate was added dropwise to N, N'- dimethyl formamide solution, water bath, recrystallized, and dried in vacuo to give β- i.e. reduction of 3,5-dibromo-mercaptoethylamine salicylaldehyde Schiff base nickel complex; contraction take β- mercaptoethylamine 3,5-dibromo-salicylaldehyde Schiff base complexes of nickel, was dissolved in absolute ethanol, was added to the nano-gold sol. Preparation of the present invention is simple, for label-type h-IGg impedance immunosensor h-IGg content, having a lower limit of detection, and good stability and good reproducibility.