The relationship between MSP58 expression level and malignant grades of human brain glioma

Objective:To investigate the relationship between MSP58 express level and malignant grades of human brain glioma.Methods: Total of 41 cases of human brain glioma tissue which were Ⅰ-Ⅳ grades according to WHO classification standard,four human brain glioma cell lines(U251,U87,BT325 and SHG-44)and 6 cases of normal brain tissues were investigated by reverse transcription polymerase chain reaction(RT-PCR)for expression of mRNA of MSP58 and by Western blot for expression of protein of MSP58.Results:The expressions of MSP58 mRNA and protein increased as the upgrading of glioma malignant grade from Ⅰto Ⅳ.There were specially significant differences of the positive expression of mRNA and protein of MSP58 respectively between the brain glioma tissues and the normal brain tissues and among the WHO Ⅰ-Ⅳ pathological grades of glioma.Both mRNA and protein of MSP58 were highly expressed in U251,U87,BT325 and SHG44 cells.Conclusion:The results suggested that MSP58 mRNA and protein were highly expressed in brain glioma and the expression level related to the malignant grade of glioma,which may associate closely with initiation and malignancy progress of brain glioma.