Cloning and sequence analysis of VP genes of avian encephalomyelitis VR strain

The experimental avian encephalomyelitis virus (AEV) strain was obtained by means of AEV being passaged in the SPF avian embryos. According to the published sequences of VP0, VP3 and VP1 genes of AEV strain 1143, 3 pairs of primers were designed. The cloning and sequence analysis of the VP0, VP3 and VP1 genes of AEV VR strain was carried out after the VP0, VP3 and VP1 genes of AEV VR strain were amplified respectively with the 3 pairs of primers by RT-PCR. The results showed that the VP0, VP3 and VP1 genes of AEV VR strain shared respectively 95.1%,93.6% and 93.9% homology with those from AEV strain 1143, and that the deduced amino acids sequences from the VP0,VP3 and VP1 genes of AEV VR strain shared respectively 97.5%,97.5% and 99.6% homology with those from AEV strain 1143.