Effect of medium additives at AcNPV infection of insect cells on recombinant ß-galactosidase production in batch and two-stage bioreactor systems

The medium additives such as CaCl2, Glucose, frutose, glutamine, glutamate and lipids were examined to enhance recombinant s-galactosidase (s-gal) production in batch and continuous two-stage bioreactor systems. The presence of each medium additive such as CaCl2, fructose, glutamate, cholesterol and tocopherol at AcNPV infection of Sf 21 cells had an effect on improved s-gal production. The recombinant s-gal production using the infection media supplemented with a mixture of 30 mM CaCl2, 2.2 mM fructose, 4.1 mM glutamate and 0.34 mM cholesterol was increased by about 40 %.