Quantitative high-throughput screening assays for the discovery and development of SIRPα-CD47 interaction inhibitors

This work was supported in part by the Avon Foundation for Women (02-2015-045, 02- 2014-051, 02-2015-095, 02-2017-034; TWM, TLB, CLF), the National Cancer Institute (1U01CA218259-01A1; TWM, TLB, CLF, JSR) the Intramural Research Programs of NCATS (Project 1ZIATR000053-03, JI) and NCI (Project ZIASC009172, DDR), the Maryland Industrial Partnerships Program (MIPs, #5914; EDG, TWM) and Cooperative Research and Development Agreement 02921 with the National Institutes of Health. This work was also conducted at the Advanced Light Source (ALS), a national user facility operated by Lawrence Berkeley National Laboratory on behalf of the Department of Energy, Office of Basic Energy Sciences, through the Integrated Diffraction Analysis Technologies (IDAT) program, supported by DOE Office of Biological and Environmental Research. Additional support comes from the National Institute of Health project ALS-ENABLE (P30 GM124169) and a High-End Instrumentation Grant S10OD018483. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.