Cloning and Bioinformatics Analysis of Swine Leukocyte Antigens ClassII Genes in Guangxi Bama Minipigs

The study aimed to elucidate the structures of SLA Class Ⅱ genes of Guangxi Bama Minipigs and the potential in pigs to humans xenotransplantation.4 specimens of ear tissues were sampled from four Guangxi Bama Minipigs,and the classical swine leukocyte antigens class Ⅱ genes(SLA-DRA,SLA-DRB1,SLA-DQA,SLA-DQB1) were amplified by PCR after reverse transcription.Purified products were cloned into pMD18-T vectors and bi-directional sequenced.Analyzing obtained sequences by BLAST in NCBI,a total of eight alleles were found,and five alleles have been found in previous reports,the other three were novel.From the homology analysis between alleles of SLA and HLA in GenBank and phylogenetic trees analysis,the results showed that the Guangxi Bama Minipigs possessed high homologous in MHC information with human,and indicated that Guangxi Bama minipigs would have potential utilization values in the study of pig to human xenotransplantation.