Method for preparing re-cellularized biological valve material

The invention relates to a method for preparing a cellularized biological valve material, which is characterized by comprising the following steps: performing cell removal treatment for a heart valve of a pig aorta, then using nordihydroguaiaretic acid solution to cross link, and performing post treatment after the cross linking. The concentration of the used cross linking agent is 0.1 to 10 mg/ml. The cross linking conditions comprise that the shake cross linking is carried out for 2 to 240 hours at 60 to 360rpm at the temperature of between 1 and 40 DEG C, then the heart valve is taken out and soaked into D-Hanks solution, and the heart valve is post-treated for 3 to 40 days at the temperature of between 1 and 6 DEG C so as to obtain the cellularized biological valve material. By adjusting the concentrations of the cross linking agent the nordihydroguaiaretic acid solution, the cross linking time, the cleaning time and the like, the adjustment and control in the aspects of cross linking mechanical strength, enzymolysis resistance, cross linking agent release, tissue regeneration capacity promotion and the like are realized.