Remedial effect of Phyllanthus acidus against Bleomycin provoked Pneumopathy

Summary The model most often used to study the pathogenesis of pulmonary fibrosis in the Bleomycin model (BLM) induced lung fibrosis, several treatments has been efficacious but not in the clinic. To examine the effect of Phyllanthus acidus against Bleomycin induced pulmonary fibrosis in rats the methanol extract prepared by soxhlet extraction method and used for the animal study. The animals received single intra3tracheal injection of Bleomycin (2.5 U/kg). The treatment animals received (500 mg/kg) of Phyllanthus acidus extract by oral gavages for a week prior after BLM treatment and Hesperidin (200 mg/kg) used as standard drug. Animal killed by lethal dose of sodium pentobarbital. The lung was removed the biochemical estimation carried out in lung homogenate. The total cell count, differential cell count, total protein, Alp, TNF3α level estimated in lung BALF. The DNA damage, histopathological changes assessed in lung tissue. The depletion of SOD, CAT, GPx, level in Bleomycin treated animal compare to control. The herbal drug regulate the normal level of enzymatic antioxidant due to free radical scavenging activity, it also control the lipid peroxidation and decrease MDA level. The plant extract regulate the normal level of total cell counts and differential cell counts. The PAE normalize the level of protein, Alp, TNF3α in the BALF compared to standard drug. The plant extracts scavenging ROS reduce DNA damage and tissue damage.