Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced byBothropstoxin-I (Lys49 Phospholipase A2) Myotoxin
2017
Purpose: Bothrops
snakes are responsible for more than 70 % of snakebites every year in Brazil
and their venoms cause severe local and systemic damages. The pharmacological
properties of medicinal plants have been widely investigated in order to
discover new alternative treatments for different classes of diseases including
neglected tropical diseases as envenomation by snakebites. In this work, we
have investigated the ability of Vochysia
haenkeana stem barks extract (VhE) to
neutralize the neuromuscular effects caused by Bothropstoxin-I (BthTX-I), the
major phospholipase A2 (PLA2) myotoxin from B. jararacussu venom.
Methods: The biological compounds of VhE were analysed
under thin layer chromatography (TLC) and its neutralizing ability against
BthTX-I was assessed through twitch-tension recordings and histological
analysis in mouse phrenic nerve-diaphragm (PND) preparations. The antimicrobial
activity of VhE was assessed against S.
aureus, E. coli and P. aeruginosa
strains. The aggregation activity of VhE was analysed under protein
precipitation assay.
Results: VhE showed the presence of phenolic compound
visualized by blue trace under TLC. VhE abolished the neuromuscular blockade
caused by BthTX-I applying the pre-toxin incubation treatment and partially
neutralized the BthTX-I action under post-toxin incubation treatment; VhE
contributed slightly to decrease the myotoxicity induced by BthTX-I. The
neutralizing mechanism of VhE may be related to protein aggregation. VhE showed
no antimicrobial activity.
Conclusion: V. haenkeana extract
which has no antimicrobial activity exhibited neutralizing ability against the
neuromuscular blockade caused by BthTX-I and also contributed to decrease its
myotoxicity. Protein aggregation involving phenolic compounds may be related in
these protective effects.
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