Carbonyl reductase gene, codase, vector, engineering bacterium and application thereof

The invention discloses a recombinant carbonyl reductase gene derived from Burkholderia gladioli ZJB-12126 and a codase thereof, a recombinant vector containing the gene, a recombinant gene engineering bacterium converted from the recombinant vector and application in catalyzing asymmetric reduction of prochiral carbonyl compound. 2-benzoylaminomethyl-3-one butyrate, tert-butyl (R)-6-cyano-5-hydroxy-3-carbonyl hexanoate and ethyl 4,4,4-trifluoro acetoacetate used as substrates are subjected to biological catalytic reaction to prepare high-optical-purity (2S,3R)-2-benzoylaminomethyl-3-hydroxy-butyrate, tert-butyl 6-cyano-(3R,5R)-dihydroxy hexanoate and ethyl (S)-4,4,4-trifluoro-3-hydroxy-butanoate. The recombinant Escherichia coli can be used as a biocatalyst to perform the biological catalytic reaction, thereby providing an alternative new enzyme source for biological catalytic synthesis of the drug chiral intermediate.