Geleneksel Türk Süt Ürünü Kaymaktan Escherichia coli O157'nin İlk Bildirimi

The aim of this study was to isolate the E. coli O157:H7/H- serotype from traditional Turkish dairy product kaymak sold in Afyonkarahisar, investigate the Stx1, Stx2, ehlyA and eaeA genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. For this purpose, a total of 100 kaymak samples sold in public bazaars located center town and villages of Afyonkarahisar were collected. E. coli O157 was isolated from 3 of 100 samples by using conventional culture methods and serological confirmation tests. The presence of Stx1, Stx2, ehlyA and eaeA genes in the isolated 3 strains was investigated by PCR. While all of 3 strains harboured the Stx1 and ehlyA genes, the Stx2 and eaeA genes were not found in the strains. The antibiotic resistance of strains was investigated by Kirby-Bauer disc diffusion test. While all of strains were resistant to cephazoline, cefoxitin and ceftiofur, the resistance was also determined in the strains to ampicillin (66.7%), cephalothin (66.7%), ceftriaxone (33.3%), nalidixic acid (33.3%) and trimethoprim/sulphamethoxazole (33.3%). The present study is the first investigation showing the presence of E. coli O157 and its virulence genes in the kaymak in Turkey.