Development of eight-plex microsatellite PCR for parentage control in deer.

Nine loci have been compiled into two multiplex microsatellite polymerase chain reaction (PCR) sets (four and five loci) and used as a tool to determine the most probable hind for each calf. The two sets were suitable to combine them in an eight-plex reaction. The exclusion probabilities of the eight-plex reaction and the nine loci were 99.3 and 99.6 % respectively, which allows the routine application of eight loci in wildlife management – as a first attempt to use molecular genetic information for such a task and it eliminates multiple sample handling in consecutive PCRs. Two loci out of the nine were never been used in deer previously.