Production of recombinant human serum albumin HSA by fermentation

The invention discloses a method to produce the recombination human serum albumin HSA by temperature-changing fermentation, comprising two process of culturing the yeast or the Pichia pastoris and fermentation. The fermentation includes two stages, the first stage is the HSA production which the temperature is 27-30DEG C, when the OD600 decreases to 200, the temperature will be decreased; and the second stage is the HAS induction period in which the temperature is 18-23DEG C and the inducing agent carbinol is added. According to the invention, temperature-changing fermentation and specific inducing agent are adpoted, the HSA expression yield of host cells has improved greatly, and the coloring degree of albumin is reduced, thus making it possible for industrial production.