Immunocharacterization of the mucin-type proteins from the intracellular stage of Trypanosoma cruzi

The surface of Trypanosoma cruzi is covered by different groups of mucins that are differentially expressed during the parasite life cycle. Wehavepreviouslyidentifiedthemajormucinsfromthebloodstreamtrypomastigotestage.Here,wepresentadditionalevidencethattogether with our previous observations allows for the identification of a second mucin group also expressed in the mammal-dwelling stages, but predominant in the intracellular amastigote.These mucins are encoded by many genes, are mostly composed of tandem repeats and are highly conserved except for an exposed hypervariable (HV) N-terminal peptide.Antibodies against HV-peptides are restricted to ~50% of the chronically infected human population, are monospecific (i.e. directed towards a single HV), and display low-avidity. In contrast, immunization with a single HV-peptide triggers high-avidity, cross-reacting humoral responses against multiple HV sequences, but not against other T. cruzi surface antigens. The diversity present in the HV regions and the characteristics of the antibody response against them suggest a role of these molecules in eluding and/or modulating the mammalian host immune system. © 2005 Elsevier SAS. All rights reserved.