Immune response induced by recombinant HPV_(16) L1-E7 in adeno-virus vector and in plasmid

To study the immune response of BALB/c mice immunized by HPV_(16) L1-E7 recombinant adeno-virus type 5 (rAd_(5) HPV_(16) L1-E7) and HPV_(16) L1-E7 instetel plasmid. rAd_(5) HPV_(16) L1-E7 virus was propagated in 293 cells line and then rAd_(5) HPV_(16) L1-E7 plasmid was prepared. The BALB/c mice were immunized with two vaccines by intramusclar injection or nasal drip. The serum IgG level of these mice was tested. The spleen cells (were) cultured. T-cell proliferation test was done and IFN-#gamma# in supernatant cultured of mice spleen cell culture was tested. The level of serum IgG in the immunized BALB/c mice was higher than that in the control mice (P<0.05). The data of IFN-#gamma# level and T-cell proliferation indicated that the immunized mice by different immuno-paths was higher than the controls (P<0.05).