Antibody Display Systems

Antibodies are promising tools for biomedical applications. With respect to functionality, scientists nowadays have a plethora of options to tailor-make therapeutic antibodies with prescribed properties. Besides hybridoma technology, which is still state of the art for antibody hit discovery but that also has intrinsic limitations regarding throughput, several other promising methodologies emerged. All of these have in common that they couple the phenotype, i.e., the binding properties of an antibody to its genotype, the genetic information that encodes for that particular moiety. In this chapter, the reader will learn about the most commonly applied technologies for antibody hit discovery.