Evaluation of α-amylase activity and falling number around maturity for soft white and soft red wheat varieties in Michigan

White wheat is, categorically, more susceptible to pre-harvest sprouting (PHS) than red wheat. Physiological maturity (PM), defined as when the seeds reach their maximum dry weight, is a critical time before harvesting. The objective of this study was to determine a reference level of α-amylase activity and the corresponding Falling Number (FN) value near the time of PM of selected red and white cultivars in the absence of PHS inducing conditions. Twenty-four soft winter wheat genotypes (12 red and 12 white) adapted to Michigan with varying historic levels of susceptibility to PHS were planted in an α-lattice design in two locations from 2008 to 2010. Spikes were collected three days before PM, at PM, and three days post PM. Samples were freeze-dried, threshed, milled and evaluated for α-amylase activity and FN value using high throughput method. Within genotype, clear trends were observed in the reduction of α-amylase activity and the increase of FN value during the physiological maturation. A nonlinear ...