Method for preparing high-purity natural L-alpha-glycerylphosphorylcholine

The invention discloses a method for preparing high-purity natural L-alpha-glycerylphosphorylcholine (L-alpha-GPC), and belongs to the technical field of phosphatides products. The method comprises the following preparation steps: taking an alkaline hydrolysis reaction solution as a raw material, directly performing alumina column chromatography, then using cation exchange resin column chromatography to remove residual choline, salts and other impurities, and performing active carbon adsorption and concentration on the obtained chromatographical liquid, so as to finally obtain the product. The obtained product has the purity of 99.0% or more through detection by high performance liquid chromatography-evaporative light scattering detector, and accords with purity demands of medicines and health products. According to the technical scheme, a new production technological method is provided for separation purification of L-alpha-glycerylphosphorylcholine, also application of alumina column chromatography in phosphatides deep processing field is increased, and also safety of L-alpha-glycerylphosphorylcholine as a bulk drug and a health product is guaranteed.