Ganoderan extraction method and ganoderan use

2012 
The invention discloses a ganoderan extraction method and a ganoderan use. The ganoderan extraction method utilizes an ultrasonic enzyme method to realize ganoderan extraction and comprises ganoderma lucidum crushing, extraction, ultrafiltration, condensation and ethanol precipitation separation. The ganoderan extraction method has a ganoderan extraction rate of 3.14 to 4.21%. The ganoderan obtained by the ganoderan extraction method has molecular weight less than 10000 dalton and is light brown. The ganoderan extraction method has simple extraction separation processes and is suitable for industrial production. Ganoderan has strong antioxidant activity and anticancer activity. In a DPPH. free radical system, IC50 of ganoderan is in a range of 0.28 to 0.43mg/mL and an ORAC value of ganoderan is in a range of 1207.66 to 1777.90 micromole Trolox/g. When a concentration of ganoderan is 2mg/ml, ganoderan has a human lung cancer cell A549 inhibition rate of 53.04 to 68.37 and can be used for preparation of an anticancer drug.
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