Quantitative estimation of garlic oil content in garlic oil based health products

Abstract The effective carbon number (ECN) concept can estimate FID response factors of garlic sulphides with good accuracy and the incorporation of such factors is necessary for the precise quantitative analysis of garlic sulphides. A GLC procedure using C 18 cartridge liquid solid phase extraction was developed to analyse sulphide content in garlic oil based health products. Diallyl disulphide, allyl methyl trisulphide and diallyl trisulphide were found to be the three major sulphides, contributing about 600 mg g −1 in the oil. The garlic oil based health products examined in this investigation had a very similar sulphide profile to that of pure garlic oil. The total garlic oil content in these products may thus be estimated by relating individual sulphide weight per cent in the products to their composition in garlic oil. Such an estimation was found to be useful and reliable in order to check the manufacturer's label claims of garlic oil contents.