Novel in vitro assays for the characterization of EMT in tumourigenesis

Vincent Koo,Amgad El Mekabaty,Peter Hamilton,Perry Maxwell,Osama Sharaf,James Diamond,Jenny A. Watson,Kathleen Williamson

Novel in vitro assays for the characterization of EMT in tumourigenesis

2010

Vincent Koo
Amgad El Mekabaty
Peter Hamilton
Perry Maxwell
Osama Sharaf
James Diamond
Jenny A. Watson
Kathleen Williamson

Background: Two novel assays quantifying Epithelial to Mesenchymal Transition (EMT) were compared to traditional motility and migration assays. TGF-β1 treatment of AY-27 rat bladder cancer cells acted as a model of EMT in tumourigenesis.

Keywords:

Cytological Techniques
Biology
Cancer cell
Pathology
Motility
Epithelial–mesenchymal transition
Migration Assay
Rat Bladder
Neoplastic Processes
In vitro toxicology
Cell dedifferentiation

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