ANTIBACTERIAL ACTIVITY OF EXTRACTS FROM SALVIA OFFICINALIS AND ROSMARINUS OFFICINALIS OBTAINED BY SONICATION AND MACERATION METHODS

Objective: Our study attempts to compare the phenolic contents and the antibacterial activity of the methanol extracts obtained by sonication and classical maceration from Rosmarinus officinalis and Salvia officinalis. Methods: The extracts were obtained from two plants belonging to Lamiaceae family; S. officinalis and R. officinalis. These plants were collected at February 2011 in the National Institute of Medicinal and Aromatic Plants (Morocco). The total phenolic content was evaluated using Folin Ciocalteu reagent. Furthermore, the antibacterial effect of the extract was tested against eight bacterial strains using the disk-diffusion method. Results: Our results show that sonication was more effective for the extraction of phenolic compounds than the classical maceration. Moreover, all extracts showed antibacterial activity against Gram-positive bacteria tested (Bacillus subtilis and Staphylococcus aureus). Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) were found to be resistant.