Sequential IgE-epitopes of ovomucoid exposed by glutathione are relevant in persistent egg allergy

Methods Reduced ovomucoid in raw eggwhite was detected using a fluorescence-labeled alkylation probe. The common antioxidants glutathione and cystein were used for in vitro linearization and effects were monitored by CD-spectrometry. Egg-allergic patients were tested serologically (n=19) and by skin prick test (n=9) for IgE against native and linearized ovomucoid and optionally its cooked state.