Influence of poisonous gases on the bio-oxidation of coalmine gas

Carbon monoxide (CO), hydrogen sulphide (H2S) and sulphur dioxide (SO2), which commonly appear and mix in coalmine gas, were investigated in terms of their effects on methane oxidation of methanotroph systems using single-factor and multi-factor experiments. The single-factor experiments that were performed showed that the methane oxidation rate and methane monooxygenase (MMO) activity of the test group at different concentrations had no evident variability when compared with the control group. Multi-factor experiments indicated that a mixture of CO, H2S and SO2 at various concentrations slightly inhibited the methane oxidation process of the methanotroph system. A pairwise comparison on the basis of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles demonstrated a high Jaccard index (ranging from 79 to 93%) similar to that of the methanotroph system, while the existence of CO, H2S and SO2, did not have evident effects on methane oxidation in the methanotroph system. Th...