RFLP Standardization Report for C4/HindIII

Seven standard bands have been defined using the C4 probe (Table 1). Band 3 (19.26 kb) is only found in one sample (WT8,9017) and is probably a partial digestion product of bands 5 and 7 (Table 2 and Fig. 1). Band 4 is found in all nondeletion haplotypes and constitutes the 3’ half of the C4A gene. In many C4A deletion haplotypes (1), (lanes 13,15, and 31: WS.ID. 9022,9023, and 9088, respectively) st. band 4 is replaced by an 8.0 kb band (st. band 6), which is produced as a result of the fusion of the C4A and C4B genes.