Analysis and regulation of the secY gene from Streptomyces griseus N2-3-11 and interaction of the SecY protein with the SecA protein

Abstract The chromosomal region encoding the secY gene of Streptomyces griseus N2-3-11 was cloned and analyzed. The secY gene encodes a polypeptide of 438 aa with a molecular mass of 47.5 kDa. The transcriptional start point of the secY gene was determined. Northern blot analysis revealed a growth phase-dependent secY expression supporting our previous findings for secA gene expression in S. griseus . Overproduction of the SecY protein was obtained when using Streptomyces lividans TK23 as host. The interaction of the SecY proteins of S. griseus , S. lividans , and Escherichia coli , respectively, with the purified SecA protein of S. griseus was demonstrated for the first time by using ligand affinity blot assays.