Gas-sensing internal enzyme fiber optic biosensor for hydrogen peroxide.

Abstract Feasibility is demonstrated for a novel gas-sensing, internal enzyme biosensing scheme for the selective measurement of hydrogen peroxide. Two horseradish peroxidase catalysed reactions are evaluated for the detection of hydrogen peroxide as it crosses a microporous Teflon membrane at 37°C. The rate at which hydrogen peroxide crosses the membrane is determined by either a fluorescence or chemiluminescence measurement and this rate is related to the concentration of hydrogen peroxide in the sample solution. Detection limits of 0.7 m M and 10 μ M are estimated for the fluorescence and chemiluminescence methods, respectively. Selectivity is demonstrated for hydrogen peroxide over ascorbic acid, uric acid and tyrosine.