Quantitative real-time PCR : methods and protocols

1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers Alessandro Raso and Roberto Biassoni 2. Parameters for Successful PCR Primer Design Stephen A. Bustin, Reinhold Mueller, and Tania Nolan 3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl 4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation Valentin Vautrot and Isabelle Behm-Ansmant 5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati 6. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR) Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constanca Britto, and Alice Gomes Fernandes Monteiro 7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR) Paula Maza-Marquez, Elisabet Aranda, Jesus Gonzalez-Lopez, and Belen Rodelas 8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani 9. A General Protocol for Accurate Gene Expression Analysis in Plants Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek 10. Gene Expression Analysis in Bacteria by RT-qPCR Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco 11. Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani 12. Molecular Monitoring of Chronic Myeloid Leukemia Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad 13. Normalization in Human Gliomas Tissue Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Marcia Rosangela Wink 14. qPCR Applications for the Determination of the Biological Age Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi 15. QuantStudio (TM) 12K Flex OpenArray (R) System as a Tool for High-Throughput Genotyping and Gene Expression Analysis Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato 16. Digital PCR and the QuantStudio (TM) 3D Digital PCR System Marion Laig, Christie Fekete, and Nivedita Majumdar