Echinococcus granulosus: diagnosis of human hydatid disease by the indirect haemoagglutination reaction with antigens from hydatid fluid and scoleces.

Abstract Tassi Carmelo , Dottorini Silvio , Scalise Giorgio and Geranio Nadia . 1981. Echinococcus granulosus : diagnosis of human hydatid disease by the Indirect Haemoagglutination reaction with antigens from hydatid fluid and scoleces. International Journal for Parasitology 11 : 85–88. Various antigenic fractions were prepared from sheep hydatid fluid and scoleces of Echinococcus granulosus by ammonium sulphate salting out. Sheep red blood cells were sensitized with all antigenic preparations and tested, by Indirect Haemoagglutination reaction, against 63 sera from humans with hydatid disease and 163 controls. The greatest sensitivity was obtained with the ‘0·8 M fraction’ and ‘Band 7’ from hydatid fluid. The specificity was excellent for all antigens examined.