Моделирование системы мультиплексного анализа ISSR-маркеров свиней

The system for analysis of pig DNA-markers on base of inter-simple sequence repeat (ISSR) was developed. The system is based on PCR amplification with fluorescent labeled primers ISSR-6 and ISSR-2 and the consequent multiplex laser detection of PCR products using DNA Analyzer. Created binary DNA matrix of ISSR profiles was analyzed using GenAlEx, v6.4 software. The study of large white, landrace and duroc breeds (n=47) showed the high degree of polymorphism of ISSR-markers: 80.8 per cent of 568 identified loci were polymorphic. The all of picks identified were observed with the frequency of 5 per cent and thus were informative. The minimal number of picks was identified in landrace breed: 199 and 195 for ISSR-6 and ISSR-2 primers, respectively. The number of identified picks in large white breed was 214 and 274 and in duroc breed 210 and 291, respectively. The landrace breed was characterized by minimal heterozygote level 23.3 per cent whereas the values of this characteristic in large white and duroc breeds were 26.7 and 27.0 per cent, respectively. The private picks were identified in each breed analyzed: their number in large white, landrace and duroc breeds was 15, 36 and 54, respectively. The developed system of ISSR-analysis will be used for characteristics of allele pool of pig breeds.