Expression and Analysis of PRL-3 in Prostatic Carcinoma Tissues

To detect the expression of PRL-3 relationship with tumor occurrence and metastasis in prostatic carcinoma tissues.RT-PCR and Western blot were used to detect the expression of PRL-3 in prostatic carcinoma tissues and paired paratumor normal tissues from 52 PCA patients,metastatic lymph nodes from 12 cases with metastasis.It is resulted that PRL-3 level was increased in primary PCA tissues as compared with paired paratumor normal tissues(PP0.05).However,the PRL-3 levels of metastatic lymph nodes in 12 cases were significantly high compared with the matched primary lesions,negative-lymph nodes and normal tissues(P0.01).There was significant relation of the expression of PRL-3 with the clinicopathological features including degree of differentiation and metastatic(P0.05),but no relation with age,family medical history,PSA was found(P0.05).It is conclused that PRL-3 gene plays an important role in metastasis and carcinogenesis of PCA,strongly suggesting that the leve of PRL-3 can be used to indicate the potential of metastasis