QUANTITATIVE DETERMINATION OF β - SITOSTEROL FROM STEM BARK OF KIGELIA PINNATA LINN.

Kigelia pinnata grows to a medium size, with short thick trunk & horizontally spreading distorted branches, covered with thick & rough grayish - brown bark. Quantitative determination of β–sitosterol was undertaken to provide an easy and simple analytical method, which can be used as a routine quality control method. HPTLC was performed using chloroform as mobil phase. The detection and quantification was performed at a wavelength 254 nm. Linearity of detector response for β–sitosterol was between the concentrations 0.005% to 0.02%. The correlation coefficient obtained for the linearity was 0.994. The recovery value of standard β– sitosterol was 92.55%. The percentage of β–sitosterol in the stem bark was found to be 0.0462 %