Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication.

DNA replication requires re-establishment of chromatin structure, which involves incorporation of newly synthesized histones. Here, Klimovskaia et al. show that phosphorylation of the histone chaperone Asf1 by Tousled-Like Kinase stimulates its ability to bind histones, thus promoting chromatin assembly.