KAR1-MEDIATED TRANSFORMATION OF BREWING YEAST
1991
Transfer of plasmid between nuclei has been observed in heterokaryons obtained from kar1 × KAR1 crosses3. On this basis, brewing yeast strain CC45 was forced to mate with K5-5A, a kar1 laboratory strain bearing plasmid YEpCR21. This multicopy plasmid carries the gene cyh2 and determines a dominant resistance to cycloheximide.
Heteroplasmons having a CC45 nucleus and hosting YEpCR21 were obtained. Plasmid stability and brewing performance have been studied.
The utility of kar1-mediated plasmid transference as an alternative method to transform brewing yeast is discussed. Advantages of YEpCR21 as a vector cloning for brewing yeast are considered as well.
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