Anatomical Phase Extraction (APE) Method: A Novel Method to Correct Detrimental Effects of Tissue-Inhomogeneity in Referenceless MR Thermometry-Preliminary Ex Vivo Investigation.

Purpose We present a novel background tissue phase removing method, called anatomical phase extraction (APE), and to investigate the accuracy of temperature estimation and capability of reducing background artifacts compared with the conventional referenceless methods. Methods Susceptibility variance was acquired by subtracting pretreatment baseline images taken at different locations (nine pretreatment baselines are acquired and called φ1 to φ9). The susceptibility phase data φS was obtained using the Wiener deconvolution algorithm. The background phase data φT was isolated by subtracting φS from the whole phase data. Finally, φT was subtracted from the whole phase data before applying the referenceless method. As a proof of concept, the proposed APE method was performed on ex vivo pork tenderloin and compared with other two referenceless temperature estimation approaches, including reweighted l1 referenceless (RW- l1) and l2 referenceless methods. The proposed APE method was performed with four different baselines combination, namely, (φ1, φ5, φ2, φ4), (φ3, φ5, φ2, φ6), (φ7, φ5, φ8, φ4), and (φ9, φ5, φ8, φ6), and called APE experiment 1 to 4, respectively. The multibaseline method was used as a standard reference. The mean absolute error (MAE) and two-sample t-test analysis in temperature estimation of three regions of interest (ROI) between the multibaseline method and the other three methods, i.e., APE, RW- l1, and l2, were calculated and compared. Results Our results show that the mean temperature errors of the APE method-experiment 1, APE method-experiment 2, APE method-experiment 3, APE method-experiment 4, and RW- l1 and l2 referenceless method are 1.02°C, 1.04°C, 1.00°C, 1.00°C, 4.75°C, and 13.65°C, respectively. The MAEs of the RW- l1 and l2 referenceless methods were higher than that of APE method. The APE method showed no significant difference (p > 0.05), compared with the multibaseline method. Conclusion The present work demonstrates the use of the APE method on referenceless MR thermometry to improve the accuracy of temperature estimation during MRI guided high-intensity focused ultrasound for ablation treatment.