Impairment of homologous recombination directed DNA repair in prostate cancer cells exposed to anchorage independence

9638 Background: The majority of patients with androgen dependant prostate cancer develop a transition to highly invasive metastatic disease, which is refractory to hormonal therapy. This complication relies on the acquisition of new properties, which allow cancer cells to invade other tissues. One mechanism via which rapid genetic changes could occur is a failure of systems controlling stability of the genome, including DNA repair. In parallel, metastatic cells are temporarily exposed to anchorage-independence -a condition in which cellular interactions with extracellular matrix proteins are restricted. The purpose of this study was to evaluate whether and how anchorage-independence could predispose prostate cancer cells to develop genomic instability. Methods: DNA repair mechanisms: non-homologous end joining (NHEJ) and homologous recombination directed DNA repair (HRR) were evaluated in human prostate cancer cell lines: DU145, LNCaP and PC-3. Cell survival was evaluated by trypan blue exclusion, cell c...