Evaluation of the accuracy of precipitation methods for HDL cholesterol determination

Several methods for HDL-Cholesterol determination have been proposed; the over-floating cholesterol evaluation after non-HDL lipoproteins precipitation by polyanions with bivalent cations or neutral polymers is practical and not expensive, but it's accuracy and precision must be strictly control led. The Authors, after a review and a quality-check of the most used methods, conclude that the best accuracy for HDL-Cholesterol determination is provided by Dextransulphate-Mg-chloride and Phosphotungstate-Mg-chloride at pH 7,5.