Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC

Objective:To develop a reserved-phase HPLC method for the content determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.Method: The analysis was carried out with Kromasil C18(4.6 mm×250 mm,5 μm) column and the mobile phase consisted of acetonitrile-1.0% phosphoric acid aqueous solution(50∶50) at a flow rate of 0.8 mL·min-1.The detection wavelength was 319 nm and the column temperature was set at 30 ℃.Result: The calibration curves were linear within the range of 0.716-7.160 μg(r=0.999 6) for polygonin and 0.740-7.400 μg(r=0.999 8) for resveratrol.The average recoveries were 99.7% for erbascoside and 98.5% for resveratrol.Conclusion: The method is simple,accurate,reproducible and precise,and applicable for the determination of polygonin and resveratrol in different age root of Polygonum cuspidatum.