AhMTII mRNA Expression Changes of Peanut Nutritorium (Arachis hypogaea L.) with Different Cadmium Absorption under Cadmium Treatment

Different vegetative organs of peanut seedlings cultured with nutrient solution containing Cd2+ were collected for testing the changes of metallothionein type Ⅱ(AhMTII) mRNA level.PMD18T-AhMTⅡ and PMD18T-actin plasmids were constructed,and then AhMTⅡ mRNA level was analyzed using SYBR Green I real-time quantitative PCR.The result indicated that the AhMTⅡ mRNA level of different tissues showed significant(P0.01) difference: root leaf stem,and the AhMTⅡ mRNA content in root of low cadmium accumulation peanut variety XD1011 was obviously lower than that in cadmium accumulation peanut variety XA004.AhMTⅡ mRNA expression had high correlation with cadmium levels,especially in the roots and leaves.Under 0~50 μmol/L cadmium levels,the R2 of XA004 and XD1011′s roots were 0.977 and 0.96,respectively and the R2 of XD1011′s leaf is 0.91;the R2 of XA004 in leaf is 0.959 under 0~200 μmol/L cadmium level.The above results would provide theoretical and technical support for screening peanut varieties with low cadmium absorption.