A Study on Extraction and Application of Polyphenol Oxidase from Sugarcane Juice

Extraction and application conditions of polyphenol oxidase (PPO) of sugarcane were studied. The best extraction conditions of polyphenol oxidase of sugarcane were obtained through experiments: Sugarcane refrigerated at 4℃,pH 6.2 disodium hydrogen phosphate potassium dihydrogen phosphate buffer was used,the concentration of polyvinyl pyrrolidone (PVPP) was 2%,the ratio (v/v) of phosphate buffer and material was 1.0. Partially purified enzyme solution was made by extraction,centrifugation,grading salting-out and dialysis. The purification multiple was 2.51,and the activity recycling was 34.9%. With catechol as substrate,the maximum absorption wavelength of enzymatic reaction products of sugarcane PPO was 410 nm. The enzymatic browning of juice can be effectively slowed down by advancing prelime and increasing pH to more than 7.5 and the pigment formation can be reduced at the beginning of the process.