Die Bedeutung von Monocyte Chemotactic Protein - 1 (MCP - 1) für die Angiogenese des Glioms

Gliomas are well vascularized tumors. Turmorangiogenese is strongly affected by angiogenesis factors. This shows a pronounced angio- and Venogenesis of experimental MCP-1 producing CNS-1 rat glioma cells by CAM assays of Gallus gallus domesticus and Coturnix coturnix japonica. Histological cut and staining techniques show the vessel differentiation of MCP-1 gliomas significantly exceeded that of control gliomas. The smooth muscle-actin is in a significantly higher proportion in the MCP-1 producing gliomas. MCP-1 glioma cells also have a higher affinity to the median of the vessels and show a stronger tumor growth, with growth in vitro is adversely affected. A possible direct effect of MCP-1 on endothelial cells and smooth muscle cells has not been established. Proliferation assays with MCP-1 in smooth muscle cells and HUVECs show significant proliferation benefits through MCP-1 for smooth muscle cells. RT-PCR show the presence of MCP-1 receptors CCR 1 and CCR-2 on both cell lines. The expression of CCR-2 mRNA was increased in both cell lines by MCP-1 stimulation. The tumor cells with rMCP-1 production, however, showed a lower mRNA expression of both rat MCP-1 receptors CCR 2 and CCR-4. A direct influence of MCP-1 on endothelial and smooth muscle cells seems possible. The exact mechanism of tumor angiogenesis by MCP-1 remains unclear. The CAM has been successfully established as a tumor angiogenesis model for glioma.