A Modified Commercial Ultrasound Scanner used for In-Vivo Photoacoustic Imaging of Nude Mice Injected with Non-Targeted Contrast Agents
2008
Photoacoustic (PA) experiments were performed using a modified commercial ultrasound scanner equipped with an
array transducer and a Nd:YAG pumped OPO laser. The contrast agent SIDAG (Bayer Schering Pharma AG, Germany),
used to enhance the optical absorption, demonstrated an expected pharmacokinetic behavior of the dye in the tumor and
in the bladder of the nude mice. A typical behavior in the tumor consisted of an initial linear increase in PA signal
followed by an exponential decay. PA signal approached the pre-injection level after about one hour following the dye
injection, which was consistent with the behavior for such contrast agents when used in other imaging modalities, such
as fluorescence imaging. The in-vivo spectral PA data from the mouse bladder, conducted 1.5 hours after the dye injection, clearly demonstrated presence of the dye. The multi-spectral PA data was obtained at 760nm, 784nm and 850nm laser excitations. The PA
intensities obtained at these three wavelengths accurately matched the dye absorption spectrum.
In addition, in the kidney, a clearance organ for this contrast agent, both in-vivo and ex-vivo results demonstrated a
significant increase (~ 40%) in the ratio of PA signal at 760nm (the peak of the dye absorption) relative to the signal at
850nm (<1% absorption), indicating significant amounts of the dye in this organ.
Our initial results confirm the desired photoacoustic properties of the contrast agent, indicating its great potential to be
used for imaging with a commercial array-based ultrasound scanner.
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