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An Improved, Efficient Method for cDNA Library Construction of Triticum aestivum
2012
基于SMART法,优化cDNA文库构建方法.将常规SMART法中的限制性内切酶SfiI替换成BsaI,在引物两侧分别加入BsaI-XhoI和BsaI-BamHI酶切位点,仅使用BsaI单酶切即可获得两不同的粘性末端,可用于连接任意载体.应用此方法构建了小麦cDNA文库,获得2.7×104个独立克隆,文库平均长度500 bp.
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