Aqueous-phase synthesis of nanoparticles of copper/copper oxides and their antifungal effect against Fusarium oxysporum
2019
Abstract Different copper based-materials have been used for controlling some fungal and bacterial pathogens. However, the antifungal activity of the copper-based materials depends on different parameters, such as the crystal phase, synthesis route, and size of the particles. Herein a facile route synthesis method of Cu/Cu x O-NPs was achieved through the aqueous phase. The influence of NaBH 4 concentration on the phase composition was studied. The synthesized Cu/Cu x O-NPs were characterized by X-Ray diffraction (XRD), Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering. Five Cu/Cu x O-NPs with different phase composition and nanoparticle size were obtained. The antifungal activity of the synthesized Cu/Cu x O-NPs was studied in vitro against Fusarium oxysporum . The results indicate that a high percent of inhibition of radial growth (IGR) was obtained with NPs, which have a higher proportion of Cu 2 O phase and relatively smaller size particles. Furthermore, hypha morphology, membrane damage and production of reactive oxygen species (ROS) was evaluated with SEM and confocal microscopy.
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