Discriminatory analyses of climacteric syndrome patients of shen deficiency syndrome

Objective To find out a set of practical,objective, and quantitative laboratory indices of climacteric syndrome (CS) patients of Shen deficiency syndrome (SDS), thus studying the essence of SDS from the perspective of laboratory medicine. Methods Recruited were 40 CS patients of SDS (or of SDS as main syndrome) as the SDS group, while another 40 healthy subjects were recruited as the control group. Their serum samples were collected. Serum levels of total triiodothyronine (TT3),total thyroxine (TT4),free triiodothyronine (FT3),free thyroxine (FT4),thyroid stimulating hormone (TSH),follicle-stimulating hormone (FSH),luteinizing hormone (LH), testosterone (TESTO), estradiol (E2),prolactin (PRL),progesterone (PROG), cortisol (CORT),immunoglobulin M (IgM), immunoglobulin G (IgG),Complement 3 (C3),complement hemolysis 50% (CH50), angiotensin converting enzyme (ACE),aldosterone (ALD), serum alkaline phosphatase (ALP), and bone Gla-protein (BGP) were measured by automatic electrochemical luminescence assay analyzer,automatic chemiluminescence assay analyzer,automatic biochemistry analyzer, and automatic enzyme-linked immunosorbent assay (ELISA) analyzer. The correlation between syndrome types and laboratory indices were judged by gradual discriminant analyses. Results (1) Compared with the control group,serum levels of CORT, TESTO, E2, TT3, FT3, FT4, TSH, C3, CH50, ALP, and BGP significantly decreased in the SDS group (P0.01, P0.05),while FSH, LH, and [JP2]ACE significantly increased (P0.05).(2)The index with stronger capacity for diagnosing CS patients of SDS was ranked from high to low as CH50, PROG, TSH, TESTO, BGP, CORT, and C3, with their contribution rate of the discriminant function being 95.9%. (3) Discriminant analysis equation of CS patients of SDS was Y=-25.904-0.468CH50 + 0.002PROG + 0.182TSH+ 9.690TESTO+1.015BGP+ 0.016CORT +33.581C3. Conclusions (1) CS patients of SDS were closely correlated with thyroid hypothalamus-pituitary-adrenal axis,hypothalamus-pituitary-adrenal axis, renin-renin-angiotensin-aldosterone system,the immune function, and bone formation, and etc. (2) CH50 might be of a high sensibility marker for diagnosing CS patients of SDS. (3) Discriminant analysis equations of laboratory medicine index may be used in preliminary diagnosis and auxiliary certificate of CS patients of SDS.