EVANS BLUE DYE TRANSPORT ACROSS THE GRANULOMA POUCH WALL.

SummaryEvans Blue dye was administered either intravenously or directly into the pouch 24 hours prior to the killing of rats bearing granuloma pouches. Plasma, pouch exudate, skin and liver dye concentrations were measured on various days during the development of the granuloma pouch. When the Evans Blue was administered intravenously, the exudate and plasma dye concentrations varied inversely; as the pouch aged less Evans Blue entered it, whereas the plasma Evans Blue concentration increased. The dye concentration in two major organs, liver and skin, remained relatively constant indicating that the dye-protein-complexing ability was unchanged and that the differences in plasma dye concentration were due to changes in the pouch wall. The exudate volume and also the exudate solids content (mg/ml) reached a peak on the 18th day and then gradually decreased. When the Evans Blue was administered into the pouch the plasma Evans Blue concentration increased during the latter stages of the pouch development. The...