Regeneration of plantlets under NaCl stress from NaN 3 treated sugarcane explants
2011
In this experiment, mutation induction in explants using NaN3 and subsequently, callus growth and plant regeneration under NaCl stressed conditions was assessed in some sugarcane ( Saccharum officinarum L.) cultivars (Thatta-10, CPF-237 and SPHS-19). Immature bases of leaf tips were cultured on MS 2n (MS salts, 3.0 mg L -1 ; 2,4-D, 0.5% NaN 3 ) for 6 days then sub-cultured on MS 2 (MS salts, 3.0 mg L -1 2,4-D), MS 2a (MS 2 , 25 mol m -3 NaCl), MS 2b (MS 2 , 50 mol m -3 NaCl) and MS 2c (MS 2 , 75 mol m -3 NaCl) media in dark condition. After 6 weeks, callus growth was observed to be significantly higher (72.34 ± 3.70%) in CPF-237 in the control (MS 2 ), and lowest (57.66 ± 4.34%) in SPHS-19 in MS 2d culture. Somatic embryos were induced in proliferated calluses on MS 3 (MS salts, 0.5 mg L -1 ; BAP, 0.4 mg L -1 ; kin, 0.3 g L -1 ; casein hydrolysate, 3% sucrose) medium under dark condition for 2 weeks. These calluses were sub-cultured on MS 4 (MS, 0.3 mg L -1 ; BAP, 0.2 mg L -1 ; kin, 3% glucose), MS 4a (MS 4 , 25 mol m -3 NaCl), MS 4b (MS 4 , 50 mol m -3 NaCl) and MS 4c (MS 4 , 75 mol m -3 NaCl) media. Maximum of 8.41 ± 0.36 plantlets callus -1 were regenerated in MS 4 (control) culture of Thatta-10, and 4.94 ± 0.05 plantlets of CPF-237 in 25 mol m -3 NaCl stressed plant regeneration (MS 4a ) medium. Plant regeneration on MS 4b (2.21 ± 0.17 plantlets callus -1 ) was observed in CPF-237 only. Regenerated plantlets were rooted and considered as salt tolerant in comparison to its parent cultivars. Key words: Kinetin, somatic embryos, regenerated plantlets, Saccharum officinarum.
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